SubtiBank SubtiBank
Version comparison:

2018-02-16 18:00:482025-06-02 02:35:51

description

class B penicillin-binding protein PBP 2A, required for [SW|cell wall synthesis ]during cell elongation

class B penicillin-binding protein PBP 2A, required for [SW|cell wall synthesis] during cell elongation, part of the [[protein|Rod complex]] for lateral [SW|cell wall synthesis] and control of cell diameter

locus

BSU25000

BSU_25000

geneLength

2148

2151

outlinks

bsu

BSU25000

BSU_25000

Gene

Coordinates

2,581,771 → 2,583,921

2,581,771 2,583,921

The protein

Protein family

[[protein|PbpH]]

[SW|transpeptidase family] (according to UniProt)

The protein

[SW|Localization]

extracellular (signal peptide) [Pubmed|18957862]

localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]

extracellular (signal peptide) [Pubmed|18957862]

during vegetative growth: evenly distributed along the membrane [pubmed|14731276]

localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]

Biological materials

Mutant

MGNA-C461 (yqgF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2459 NBRP B. subtilis, Japan]

BKE25000 (Δ[[gene|pbpA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG

BKK25000 (Δ[[gene|pbpA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG

MGNA-C461 (yqgF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2459 NBRP B. subtilis, Japan]

BKE25000 ([[gene|pbpA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG

BKK25000 ([[gene|pbpA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG

Labs working on this gene/protein

[SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

References

9851991, 9139922, 12896990, 18957862, 20487272, 21636744, 21636745, 21815947, 23895585, 22846910, 14731276

9851991, 9139922, 12896990, 18957862, 20487272, 21636744, 21636745, 21815947, 23895585, 22846910, 14731276, 31086310, 32152588

The protein

Paralogous protein(s)

[[protein|PbpH]]

labs

[SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]